(E) Individuals [32P]-labeled RNA fragments have been incubated to the ZFD proteins, split up on the a non-denaturing polyacrylamide gel, then envisioned from the autoradiography. (F) [32P]-labeled Inches SSCR RNA are incubated with otherwise without the ZFD necessary protein fragment regarding the lack or presence of numerous unlabeled RNAs. The fresh complexes was broke up for the a low-denaturing polyacrylamide serum then visualized from the autoradiography.